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KMID : 0606920110190030331
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Biomolecules & Therapeutics
2011 Volume.19 No. 3 p.331 ~ p.335
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Anti-Inflammatory Effects of Hexane Fraction from White Rose Flower Extracts via Inhibition of Inflammatory Repertoires
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Lee Hwa-Jeong
Kim Han-Seok
Kim Seung-Tae
Park Dong-Sun
Hong Jin-Tae
Kim Yun-Bae
Joo Seong-Soo
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Abstract
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In this study, we determined the anti-infl ammatory activity and mechanism of action of a hexane fraction (hWRF) obtained from white Rosa hybrida fl owers by employing various assays such as quantitative real-time PCR, Western blotting, and Electrophoretic- Mobility Shift Assay (EMSA). The results revealed that the hWRF had excellent anti-infl ammatory potency by reducing infl ammatory repertoires, such as inducible nitric oxide synthase (iNOS), interleukin-1β, and cyclooxygenase-2 (COX-2) in RAW264.7 cells when stimulated with lipopolysaccharide (LPS), a pro-infl ammatory mediator. The reduction of nitric oxide (NO) release from RAW 264.7 cells supported the anti-infl ammatory effect of hWRF. Interestingly, hWRF effectively inhibited LPS-mediated nuclear factor-κB (NF-κB) p65 subunit translocation into the nucleus and extracellular signal-regulated kinase (ERK)1/2 phosphorylation, suggesting that hWRF anti-infl ammatory activity may be based on inhibition of the NF-κB and MAPK pathways. Based on the fi ndings described in this study, hWRF holds promise for use as a potential anti-infl ammatory agent for either therapeutic or functional adjuvant purposes.
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KEYWORD
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Rosa hybrida, Infl ammatory repertoire, RAW 264.7, Lipopolysaccharide, Nuclear factor-κB
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